Repeated-Measures ANOVA

Goal. Test whether performance changes across four conditions measured on the same participants.

Design & Experiment

• Within-subjects factor: Condition with 4 levels (C1, C2, C3, C4).
• s = 8 participants measured in k = 4 conditions ⇒ total observations \(N = s \times k = 32\).
• Example context: the same students take four weekly quizzes after different study activities.

Figure 1: Profile plot (each subject as a line across the four conditions).

Data

Scores (rows = participants S1–S8; columns = conditions C1–C4):

Figure 2: Means ± SEM for C1–C4 (bar/line).

Step 1 — Condition Means (and sample variances)

\[ \begin{aligned} \bar X_{\mathrm{C1}} &= 573/8 = 71.625, \quad & s^2_{\mathrm{C1}} &= 4.8393 \\ \bar X_{\mathrm{C2}} &= 601/8 = 75.125, \quad & s^2_{\mathrm{C2}} &= 5.5536 \\ \bar X_{\mathrm{C3}} &= 621/8 = 77.625, \quad & s^2_{\mathrm{C3}} &= 7.6964 \\ \bar X_{\mathrm{C4}} &= 654/8 = 81.750, \quad & s^2_{\mathrm{C4}} &= 7.0714 \end{aligned} \]

Step 2 — Sums of Squares

Notation: \(s=8\) subjects, \(k=4\) conditions, grand mean \( \bar X = 76.53125\).

2A. Total

\[ SS_{\text{total}}=\sum_{i=1}^{s}\sum_{j=1}^{k}\bigl(X_{ij}-\bar X\bigr)^2 =\mathbf{611.96875}. \]

2B. Conditions (Treatment)

\[ SS_{\text{cond}}= s \sum_{j=1}^{k}\bigl(\bar X_{\cdot j}-\bar X\bigr)^2 = 8 \left[(71.625-76.53125)^2 + (75.125-76.53125)^2 + (77.625-76.53125)^2 + (81.75-76.53125)^2\right] =\mathbf{435.84375}. \]

2C. Subjects

\[ SS_{\text{subj}}= k \sum_{i=1}^{s}\bigl(\bar X_{i\cdot}-\bar X\bigr)^2 = 4 \sum_{i=1}^{8}\bigl(\bar X_{i\cdot}-76.53125\bigr)^2 =\mathbf{162.71875}. \]

2D. Error (Residual)

\[ SS_{\text{error}}= SS_{\text{total}} - SS_{\text{cond}} - SS_{\text{subj}} = 611.96875 - 435.84375 - 162.71875 =\mathbf{13.40625}. \]

Figure 3: Partitioning variance diagram (Total → Conditions + Subjects + Error).

Step 3 — Degrees of Freedom & Mean Squares

\[ \begin{aligned} df_{\text{cond}} &= k-1 = 3, \\ df_{\text{subj}} &= s-1 = 7, \\ df_{\text{error}} &= (s-1)(k-1) = 7\times3 = 21, \\ df_{\text{total}} &= sk-1 = 31. \end{aligned} \]

\[ MS_{\text{cond}} = \frac{SS_{\text{cond}}}{df_{\text{cond}}} =\frac{435.84375}{3}=\mathbf{145.28125},\qquad MS_{\text{error}} = \frac{SS_{\text{error}}}{df_{\text{error}}} =\frac{13.40625}{21}=\mathbf{0.6383928571}. \]

Step 4 — Test Statistic & p-value

\[ F = \frac{MS_{\text{cond}}}{MS_{\text{error}}} = \frac{145.28125}{0.6383928571} =\mathbf{227.5734}. \] With \(df_1=3\) and \(df_2=21\), this is extremely large. The right-tail p-value is effectively \(p \lt 10^{-12}\) (i.e., \(p \ll .001\)).

Figure 4: F distribution with observed F marked and right-tail region shaded.

Repeated-Measures ANOVA Summary Table

Interpretation

Mean performance increases steadily from C1 → C4, and the repeated-measures ANOVA shows a highly significant effect of Condition, \(F(3,21)=227.57,\, p\ll .001\). Follow-ups (e.g., paired t-tests with Bonferroni/Holm) can localize which pairs of conditions differ.

Assumptions (checklist)

• Sphericity (equal variances of the differences between condition pairs). If violated, apply Greenhouse–Geisser or Huynh–Feldt correction to \(df\).
• Approximately normal scores within each condition.
• No carryover/fatigue effects that confound order (counterbalancing helps).

Figure 5: Sphericity concept sketch (pairwise difference variances).

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